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KMID : 0882419970520040470
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Korean Journal of Medicine
1997 Volume.52 No. 4 p.470 ~ p.476
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A Study on the Calcium - Binding Protein in Gallbladder Bile
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Kim Myung-Hwan
Jung Sung-Ae
Chae Hee-Bok
Ghim Thad
Yoo Byeung-Moo
Seo Dong-Wan
Lee Sung-Koo
Jung Hwoon-Yong
Min Young-Il
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Abstract
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Objective : Calcium salts are usually present in the center of all types of gallstones, including pure cholesterol stones. It has been postulated that precipitation of calcium salts might act as a nidus for gallstone formation. Our goal was to determine whether a calcium-binding protein was present in bile and whether this protein has any calcium-binding properties.
Methods : Calcium-binding moiety was obtained by addition of CaC12 into the gallbladder bile. Calcium-binding protein was identified by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The capacity to bind calcium was confirmed by autoradiography with 45Ca++.
Results : We identified a protein(M.W.
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